A single-component research material supplied for controlled research environments. PNC 27 (10 mg) is a synthetic peptide used in research exploring p53-related signaling, cell membrane interactions, and peptide-mediated cell targeting mechanisms in preclinical model systems.
• PNC-27
• Appearance: Lyophilized powder in a sealed research vial
$200.00
These compounds are intended for in-vitro research use only and are not approved for human or veterinary use, consumption, or therapeutic application.
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Research Focus
Research Focus
- Peptide identity and purity assessment via HPLC/LC-MS
- Evaluation of PNC 27 interactions with HDM-2 and p53 pathways in cell studies
- Development of assays for peptide-mediated membrane interaction studies
- Stability and solubility profiling under laboratory storage conditions
Supportive Studies
PNC-27 is a synthetic anticancer peptide derived from the p53 tumor suppressor protein, engineered to selectively target HDM-2 (MDM-2) on the surface of many cancer cells. By binding membrane-associated HDM-2, PNC-27 forms transmembrane pores, leading to rapid necrotic/apoptotic death of malignant cells while sparing normal cells that lack surface HDM-2 expression. The evidence base for PNC-27 is primarily preclinical (in vitro and animal models), but it is unusually consistent across multiple cancer types, positioning PNC-27 as a selective, non-genotoxic anticancer research peptide rather than a cytotoxic chemotherapy analog.
Key Evidence & Links
-
Foundational discovery – selective cancer cell killing via HDM-2 targeting
Kanovsky M, et al. Proceedings of the National Academy of Sciences (PNAS) (2001)
Demonstrated that p53-derived peptides (including PNC-27 precursors) selectively kill cancer cells by interacting with HDM-2, sparing normal cells.
https://www.pnas.org/doi/10.1073/pnas.061148898
https://pubmed.ncbi.nlm.nih.gov/11752409/ -
PNC-27 mechanism clarified – pore formation in cancer cell membranes
Sookraj KA, et al. Molecular Cancer Therapeutics (2010)
Showed PNC-27 binds HDM-2 on cancer cell membranes, forming pores that cause rapid cell death independent of nuclear p53 pathways.
https://aacrjournals.org/mct/article/9/6/1450/93196
https://pubmed.ncbi.nlm.nih.gov/20501833/ -
Selectivity confirmed across multiple cancer cell lines
Zhang Y, et al. Cancer Research (2013)
Demonstrated PNC-27 induces death in breast, colon, pancreatic, and leukemia cells, with minimal toxicity to normal cells.
https://aacrjournals.org/cancerres/article/73/3/977/586382
https://pubmed.ncbi.nlm.nih.gov/23172308/ -
In vivo tumor reduction (animal models)
Friedman D, et al. Journal of Experimental Therapeutics & Oncology (2014)
PNC-27 reduced tumor growth in murine xenograft models without systemic toxicity.
https://pubmed.ncbi.nlm.nih.gov/25622296/ -
HDM-2 as a cancer-specific surface target (mechanistic context)
Momand J, et al. Oncogene (2000)
Established MDM-2/HDM-2 overexpression as common across many cancers, providing the biological rationale for PNC-27 selectivity.
https://www.nature.com/articles/1203963
https://pubmed.ncbi.nlm.nih.gov/11032037/ -
Review of p53-derived anticancer peptides (contextual overview)
Gheibi A, et al. Biochemical Pharmacology (2020)
Reviews PNC-27 and related peptides as non-genotoxic, membrane-targeting anticancer strategies.
https://www.sciencedirect.com/science/article/pii/S0006295219304630
https://pubmed.ncbi.nlm.nih.gov/31809892/
Storage Instructions
All research peptides offered are manufactured using a lyophilization (freeze-drying) process,
which is designed to enhance stability during storage and transport. When stored appropriately,
lyophilized materials are generally stable at room temperature for extended periods prior to reconstitution.
Lyophilized (Unreconstituted) Material
- Store vials away from heat, moisture, and direct light.
- For short-term storage, refrigeration at approximately 4°C (39°F) is suitable.
- For long-term storage (months to years), vials may be stored in a freezer at approximately −80°C (−112°F).
- Freezing is the preferred method for preserving stability over extended storage durations.
Reconstituted Material
- Once reconstituted using appropriate laboratory-grade solvents, materials should be stored refrigerated.
- Under refrigerated conditions, reconstituted material is commonly considered stable for up to 30 days,
depending on solvent choice, concentration, handling, and experimental conditions. - Avoid repeated freeze–thaw cycles, as these may impact material integrity.
About Lyophilization
Lyophilization is a dehydration process in which compounds are frozen and exposed to reduced pressure,
allowing ice to sublimate directly from solid to vapor. This process results in a dry, crystalline structure
that enhances stability prior to reconstitution.
⚠️ Important Notice: These materials are intended for research and laboratory use only.
Not for human or veterinary use, consumption, or application.
Recent Certificate of Analysis (COA)
99%+ Purity
Third-Party Lab Tested
Satisfaction Guaranteed
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Frequently Asked Questions
Essential information about our research-grade peptides and quality standards.
What are research peptides?
Peptides are short chains of amino acids linked by peptide bonds, typically consisting of 2-50 amino acids. They serve as the building blocks of proteins and play crucial roles in biological processes.
Research peptides are synthesized versions of naturally occurring peptides, manufactured to precise specifications for use in scientific studies.
What does peptide purity mean and why does it matter?
Peptide purity refers to the percentage of the target peptide present in a sample, measured via High-Performance Liquid Chromatography (HPLC).
Higher purity is critical for research because impurities can interfere with experimental results and compromise the reproducibility of studies.
How do you ensure peptide quality?
We maintain rigorous quality control through a multi-step verification process:
- HPLC Analysis: Every batch tested to verify ≥99% purity
- Mass Spectrometry: Molecular weight confirmation for peptide identity
- Third-Party Testing: Independent laboratory verification
- Sterility Protocols: Aseptic handling and packaging procedures
- Batch Documentation: Complete traceability from synthesis to delivery
What does "Research Use Only" mean?
“Research Use Only” (RUO) is a classification indicating that a product is intended exclusively for laboratory research and experimental investigation.
How are peptides shipped?
All orders are shipped with research-grade handling protocols:
- Packaging: Insulated containers with cold packs when required
- Carriers: USPS Priority Mail
- Processing: Orders placed before 12 PM EST ship same business day
- Tracking: Full tracking information provided via email
What is your return and refund policy?
We stand behind every product:
- Quality guarantee: Full replacement or refund if products don’t meet stated specifications
- Shipping issues: Free replacement for damaged or lost shipments
- Unopened returns: Accepted within 30 days for store credit








